Robotic Sperm Preparation in an Intelligent Automated in Vitro Fertilization and Intracytoplasmic Sperm Injection Platform
Murray et al. | Patent No. US 12,178.475 B1
A method of artificial-intelligence-based robotic pipetting for spermatozoa preparation includes positioning a vessel containing a semen sample on a staging mechanism, wherein the staging mechanism includes at least one motor to position the vessel at a specified orientation, using an artificial intelligence/machine learning (AI/ML system) sys- tem. The method includes confirming a position of the vessel, using the AI/ML system. The method includes direct- ing a robotic pipettor to place a pipette tip within the semen sample at a position specified by the AI/ML system. The method includes aspirating and mixing the semen sample using the robotic pipettor. The method includes receiving an image object from an imaging system that includes a micros- copy system, a camera system, and a lighting system. The method includes determining a semen liquefaction state of the semen sample based at least in part on the image object, using the AI/ML system.
